ABVector公司产品介绍【代理商代购现货】
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ABVector成立于2001年,是设计和开发促进蛋白质折叠的新型载体的领导者。我们是高等真核表达系统的唯一供应商,这些系统合成分子伴侣的集合,其能够帮助正确折叠大量表达的外源蛋白。我们的专利伴侣技术使我们能够以天然构象生产药物靶标,例如:与Hsp90复合的盐皮质激素受体,否则其不具有活性,因此不能从其他供应商处商购。我们提供几乎完整的治疗相关类固醇/核受体库存作为GST融合,即广泛用于高通量药物筛选的形式,例如LanthaScreen®,PolarScreen™,AlphaScreen®和LANCE™配体结合和共激活蛋白肽结合/置换试验以及闪烁邻近试验。*
任务
我们的使命是提供创新工具,以促进正确折叠的生物活性蛋白质的生产。我们计划在帮助科学界了解蛋白质结构和功能方面发挥重要作用。为实现这一使命,我们的愿景是扩大我们的技术领导地位,以涵盖主要细胞室中蛋白质折叠的大多数方面。最近,我们正在开发多种改进的基于蛋白质的分析方法。
好处
到目前为止,蛋白质表达的发展主要集中在改进产生重组克隆的便利性和提高蛋白质产生水平上。我们开发了ProFold™,这是第一个促进高等真核系统蛋白质折叠的商业化载体。ProFold™载体的主要优点是在细胞质或ER中分子伴侣的过表达,这有助于折叠感兴趣的蛋白质。我们用哺乳动物,酵母和植物蛋白测试了我们的载体,并观察到与使用常规杆状病毒载体或大肠杆菌表达的相同蛋白质相比,生物活性蛋白质的产量显着提高。使用专门用于折叠Hsp90依赖性蛋白质的多伴侣系统观察到靶蛋白表达和生物活性的显着改善。
ProFold™载体的另一个优点是通过绿色荧光法优化蛋白质表达系统。ProFold™载体提供维多利亚水母(Aequoreavictoria)绿色荧光蛋白(GFP)的表达。这允许通过\"末端绿色稀释”监测病毒繁殖,优化培养条件和滴定病毒原种。它节省了时间和精力;无需昂贵的杆状病毒滴定试剂盒或专用设备。所需的唯一必需品是相同的365nm紫外透射仪和数码相机,常用于溴化乙锭染色凝胶。此外,您可以使用荧光显微镜计算受感染细胞的绿色病灶或利用流式细胞仪。
1.Kits Clickontheproductnamefortechnicalinformation! Cat. #ProductSpecificationPricing&Ordering*Notes#K20GreenKit Greatvalue,allthevectorsandacontrolyouneedtostart Highleveltargetproteinexpressioncontrolledbyofpolyhedrinpromoter GFPMarkertosimplifymanipulations CompatIBLewithSf9,Sf21,Hi-Fiveinsectcellsandwithwiderangeoftransfectionreagentsandmediarecommendedforbaculoviruswork1. ProGreen™GFP+ baculovirusvectorDNA,25 μl,2.5 μg2. pVL1393 plasmidtransfervector,50 μl,25 μg 3. ProFectin™ transfectionreagent, 50 μl 4. GC(greencontrol) GFP+ controlrecombinantbaculovirus $490.00 #K22C2Kit Recommendedforexpressionofdifficultcytoplasmicproteinsandproteinswithlargecytoplasmicdomains1. ProFold™-C2 baculovirusvectorDNA,25 μl,2.5 μg2. pVL1393 plasmidtransfervector,50 μl,25 μg$895.00 #K24ER1-beekit Recommendedforhighlevelexpressionofglycoproteinsusingpowerfulhoneybeemelittinsignalsequence1. ProFold™-ER1 baculovirusvectorDNA,25 μl,2.5 μg2. pAB-bee™ plasmidtransfervector,50 μl,25 μg$995.00 |
2.LinearizedbaculovirusvectorDNA |
Clickontheproductnamefortechnicalinformation! |
Cat. | Product | Specification | UnitSize | Pricing&Ordering* | Notes |
#A1 | ProGreen™ CsClpurifiedBsu36IlinearizedBaculovirusvectorDNA | Highleveloftargetproteinexpression GFPmarkertomonitorviruspropagation | 25 μl,2.5 μg 5transfections |
$295.00 |
#A3 | ProFold™-C2 CsClpurifiedBsu36IlinearizedBaculovirusvectorDNA | OverexpressedHsp40andHsc70chaperonesfacilitatetargetproteinfoldinginthecytoplasm GFPmarkertomonitorviruspropagation Slightlyreducedlevelofproteinexpression | 25 μl,2.5 μg 5transfections | $695.00 |
#A7 | ProFold™-PDI CsClpurifiedBsu36IlinearizedBaculovirusvectorDNA | OverexpressedPDIfacilitatesfoldingofcysteine-richproteins Highleveloftargetproteinexpression | 25 μl,2.5 μg 5transfections | $465.00 |
#A10L | ProEasy™ CsClpurifiedlinearizedBaculovirusvectorDNA | Theeasiestgenerationofrecombinantbaculoviruses IdealforHTSapplications Highleveloftargetproteinexpression | 125 μl,12.5 μg 25transfections | $2950.00 | . |
#B2 | pAcAB3 | Highlevelexpressionofupto3targetproteinsusingasinglerecombinantbaculovirus | 50 μl,0.5 μg/μl | $395.00 |
#B3H | pAB-bee™-8xHis | ProvidestargetproteinswithpowerfulhoneybeemelittinsignalsequencetofacilitatetranslocationintotheER Recommendedforhighlevelexpressionofsecretedandtransmembraneproteins N-orC-terminal8xHistagforaffinitypurification | 50 μl,0.5 μg/μl | $495.00 |
#B4 | pAB-6xHis™ | HighlevelexpressionofproteinswithcleavableN-terminalpolyhistidinetag | 50 μl,0.5 μg/μl | $295.00 |
#B6 | pAB-MBP™ | HighlevelexpressionofproteinswithcleavableN-terminalMBPtag MBPtagimprovesproteinsolubilityandyield | 50 μl,0.5 μg/μ | $395.00 |
#B8 | pVL-GFP | HighlevelexpressionofproteinswithC-terminalGFPtag | 50 μl,0.5 μg/μ | $395.00 |
4.Controls |
Clickontheproductnamefortechnicalinformation! |
Cat. # | Product | Specification | UnitSize | Pricing&Ordering* | Notes |
#C13 | NC(negative control) Recombinantbaculovirusstock | ExtractsofcellsinfectedwithNCisanidealnegativecontrolasthereisnopolyhedrinortargetproteinexpression NCextractsarerunsidebysideinSDS-PAGEwithextractsofcellsinfectedwithrecombinantbaculoviruses RecombinantproteinisdetectedasaprominentbandwhichisabsentinNCextracts | 1ml,108 p.f.u./ml | $195.00 |
#C15 | GCP(greencontrolplasmid) PlasmidtransfervectorDNA | PositivecontrolfortransfectionwithlinearizedGFP- baculovirusDNA.Greenfluorescenceofcellsisindicativeofsuccessfultransfection. | 50 μl,0.2 μg/μl | $145.00 |
#H15 | FoldHelper™-104 Recombinantbaculovirusstock | OverexpressedHsp104tofacilitaterecyclingofaggregatedproteinsforfoldingwithchaperones | 1ml,108p.f.u./ml | $365.00 |
#H17 | FoldHelper™-57P Recombinantbaculovirusstock | OverexpressedPDIandERp57tofacilitatefoldingofcysteine-richproteins | 1ml,108p.f.u./ml | $295.00 |
6.Steroid/nuclearreceptors
a.Purifiedreceptor/Hsp90complexes
Clickontheproductnamefortechnicalinformation!
Cat. # | Product | Specification | UnitSize | Pricing&Ordering* | Notes |
#SA90 | AR-LBD | PurifiedGST-taggedhumanAndrogenreceptorLBDincomplexwithHsp90.Suitableforligandbindingandcoactivatorpeptiderecruitmentassays. | 50μg | $980.00 |
#SEb90 | ERβ-LBD | PurifiedGST-taggedhumanEstrogenreceptorbetaLBDincomplexwithHsp90.Suitableforligandbindingandcoactivatorpeptiderecruitmentassays. | 50μg | $880.00 |
#S28 | MR-LBD | PurifiedGST-taggedhumanMineralocorticoidreceptorLBDincomplexwithHsp90.Suitableforligandbindingandcoactivatorpeptiderecruitmentassays. | 50μg | $1,100.00 |
#SPX90 | PXR-LBD | PurifiedGST-taggedhumanPregnaneXreceptorincomplexwithHsp90.Suitableforligandbindingandcoactivatorpeptiderecruitmentassays. | 50μg | $1,100.00 |
#SC90 | CAR | PurifiedGST-taggedhumanConstitutiveandrostanereceptorincomplexwithHsp90.Suitableforligand-bindingandcoregulatordisplacementassays. | 50μg | $1,100.00 |
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